Labelling oligonucleotides to high specific activity (I)
نویسندگان
چکیده
منابع مشابه
Polymerase synthesis of oligonucleotides containing a single chemically modified nucleobase for site-specific redox labelling.
Enzymatic construction of single-nucleobase redox-labelled oligonucleotides was developed either based on polymerase incorporation of a single modified nucleoside triphosphate (dNTP) followed by primer extension (PEX) with natural dNTPs or based on PEX with a biotinylated one-nucleotide overhang template, magnetoseparation and the second PEX with a full-length template.
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1989
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/17.12.4605